primary hbec Search Results


cell lysates from primary human bronchial epithelial cells f508del/f508del and 2184insa/w1282x cf patients (cf-hbec)  (Epithelix)
90
Epithelix cell lysates from primary human bronchial epithelial cells f508del/f508del and 2184insa/w1282x cf patients (cf-hbec)
Cell Lysates From Primary Human Bronchial Epithelial Cells F508del/F508del And 2184insa/W1282x Cf Patients (Cf Hbec), supplied by Epithelix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lysates from primary human bronchial epithelial cells f508del/f508del and 2184insa/w1282x cf patients (cf-hbec) - by Bioz Stars, 2026-02
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primary copd human bronchial epithelial cells (hbec  (Lonza)
90
Lonza primary copd human bronchial epithelial cells (hbec
Mucociliary transport rates were determined by tracking fluorescent microspheres placed on top of cell layer of unchallenged or TNF-challenged <t>HBEC</t> ALI cultures with or without ACY-1083 treatment. (A) Images of tracked beads in the ImageJ software with the Fiji plugin Tracking. Lines show movement of beads. (B) Microsphere speeds determined from three <t>COPD</t> donors and three fields per well. Box plot with dots representing three COPD donors and whiskers showing min and max. Data was normalized to the DMSO-control without TNF for each donor (set to 100%). Statistical analysis was performed using one-way ANOVA with Dunnett&s multiple comparisons test.
Primary Copd Human Bronchial Epithelial Cells (Hbec, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary copd human bronchial epithelial cells (hbec/product/Lonza
Average 90 stars, based on 1 article reviews
primary copd human bronchial epithelial cells (hbec - by Bioz Stars, 2026-02
90/100 stars
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primary hbec cells  (Lonza)
90
Lonza primary hbec cells
a Primary human bronchial epithelial cells <t>(HBEC)</t> were exposed to Poly(I:C) (100 μg/ml) for 24 h, and the secretion of chemokines/cytokines were determined. Error bars represent S.E.M. of two independent experiments. b <t>Primary</t> <t>HBEC</t> were exposed to Poly(I:C) (100 μg/ml) for 6 and 24 h (left panel), or to cisplatin for 24 h (right panel), and the percentage of Annexin V+ cells was determined by flow cytometry. Error bars represent S.E.M. of three (left panel) and two (right panel) independent experiments. c Human immortalized bronchial epithelial HBEC3-KT cells were treated with Poly(I:C) (100 μg/ml) for 6 h and 24 h (left panel), or to cisplatin (100 μM) for 24 h (right panel), and the percentage of Annexin V+ cells was determined. Error bars represent S.E.M. of four (left panel) and three (right panel) independent experiments. ** P < 0.01. d HBEC3-KT cells were transfected with a control non-silencing siRNA (siNS) or targeting TLR3 (siTLR3), exposed to Poly(I:C) (100 μg/ml) for 24 h, and the secretion of chemokines/cytokines was determined by ELISA. Error bars represent S.E.M. of three independent experiments. * P < 0.05. e HBEC3-KT cells were transfected as in d , lysed, and immunoblotted as indicated. f The indicated cells were lysed and immunoblotted for TLR3 and actin. Representative images of three independent experiments. g HBEC3-KT cells transfected with a control siRNA (siNS) or targeting c-FLIP (siFLIP), and were treated for 1 h with BV6 (5 μM). The cells were then exposed for 6 h to increasing doses of Poly(I:C), and the percentage of Annexin V+ cells was determined. Error bars represent S.E.M. of four independent experiments. * P < 0.05 and ** P < 0.01. h HBEC3-KT cells were treated as in f , lysed, and immunoblotted as indicated. i HBEC3-KT transfected with siNS or siFLIP were then exposed to Poly(I:C) (100 μg/ml) for the indicated times in presence or absence of BV6 (5 μM). The cells were then lysed and immunoblotted as indicated. Caspase cleavage products are indicated by arrowheads. Representative images of two independent experiments
Primary Hbec Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary hbec cells/product/Lonza
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primary hbec cells - by Bioz Stars, 2026-02
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primary human hbec cells  (ScienCell)
90
ScienCell primary human hbec cells
a Primary human bronchial epithelial cells <t>(HBEC)</t> were exposed to Poly(I:C) (100 μg/ml) for 24 h, and the secretion of chemokines/cytokines were determined. Error bars represent S.E.M. of two independent experiments. b <t>Primary</t> <t>HBEC</t> were exposed to Poly(I:C) (100 μg/ml) for 6 and 24 h (left panel), or to cisplatin for 24 h (right panel), and the percentage of Annexin V+ cells was determined by flow cytometry. Error bars represent S.E.M. of three (left panel) and two (right panel) independent experiments. c Human immortalized bronchial epithelial HBEC3-KT cells were treated with Poly(I:C) (100 μg/ml) for 6 h and 24 h (left panel), or to cisplatin (100 μM) for 24 h (right panel), and the percentage of Annexin V+ cells was determined. Error bars represent S.E.M. of four (left panel) and three (right panel) independent experiments. ** P < 0.01. d HBEC3-KT cells were transfected with a control non-silencing siRNA (siNS) or targeting TLR3 (siTLR3), exposed to Poly(I:C) (100 μg/ml) for 24 h, and the secretion of chemokines/cytokines was determined by ELISA. Error bars represent S.E.M. of three independent experiments. * P < 0.05. e HBEC3-KT cells were transfected as in d , lysed, and immunoblotted as indicated. f The indicated cells were lysed and immunoblotted for TLR3 and actin. Representative images of three independent experiments. g HBEC3-KT cells transfected with a control siRNA (siNS) or targeting c-FLIP (siFLIP), and were treated for 1 h with BV6 (5 μM). The cells were then exposed for 6 h to increasing doses of Poly(I:C), and the percentage of Annexin V+ cells was determined. Error bars represent S.E.M. of four independent experiments. * P < 0.05 and ** P < 0.01. h HBEC3-KT cells were treated as in f , lysed, and immunoblotted as indicated. i HBEC3-KT transfected with siNS or siFLIP were then exposed to Poly(I:C) (100 μg/ml) for the indicated times in presence or absence of BV6 (5 μM). The cells were then lysed and immunoblotted as indicated. Caspase cleavage products are indicated by arrowheads. Representative images of two independent experiments
Primary Human Hbec Cells, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary human hbec cells/product/ScienCell
Average 90 stars, based on 1 article reviews
primary human hbec cells - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


Mucociliary transport rates were determined by tracking fluorescent microspheres placed on top of cell layer of unchallenged or TNF-challenged HBEC ALI cultures with or without ACY-1083 treatment. (A) Images of tracked beads in the ImageJ software with the Fiji plugin Tracking. Lines show movement of beads. (B) Microsphere speeds determined from three COPD donors and three fields per well. Box plot with dots representing three COPD donors and whiskers showing min and max. Data was normalized to the DMSO-control without TNF for each donor (set to 100%). Statistical analysis was performed using one-way ANOVA with Dunnett&s multiple comparisons test.

Journal: bioRxiv

Article Title: HDAC6 inhibitor ACY-1083 shows lung epithelial protective features in COPD

doi: 10.1101/2022.03.21.485098

Figure Lengend Snippet: Mucociliary transport rates were determined by tracking fluorescent microspheres placed on top of cell layer of unchallenged or TNF-challenged HBEC ALI cultures with or without ACY-1083 treatment. (A) Images of tracked beads in the ImageJ software with the Fiji plugin Tracking. Lines show movement of beads. (B) Microsphere speeds determined from three COPD donors and three fields per well. Box plot with dots representing three COPD donors and whiskers showing min and max. Data was normalized to the DMSO-control without TNF for each donor (set to 100%). Statistical analysis was performed using one-way ANOVA with Dunnett&s multiple comparisons test.

Article Snippet: Primary COPD human bronchial epithelial cells (HBEC) were purchased from Lonza (Basel, Switzerland).

Techniques: Software

a Primary human bronchial epithelial cells (HBEC) were exposed to Poly(I:C) (100 μg/ml) for 24 h, and the secretion of chemokines/cytokines were determined. Error bars represent S.E.M. of two independent experiments. b Primary HBEC were exposed to Poly(I:C) (100 μg/ml) for 6 and 24 h (left panel), or to cisplatin for 24 h (right panel), and the percentage of Annexin V+ cells was determined by flow cytometry. Error bars represent S.E.M. of three (left panel) and two (right panel) independent experiments. c Human immortalized bronchial epithelial HBEC3-KT cells were treated with Poly(I:C) (100 μg/ml) for 6 h and 24 h (left panel), or to cisplatin (100 μM) for 24 h (right panel), and the percentage of Annexin V+ cells was determined. Error bars represent S.E.M. of four (left panel) and three (right panel) independent experiments. ** P < 0.01. d HBEC3-KT cells were transfected with a control non-silencing siRNA (siNS) or targeting TLR3 (siTLR3), exposed to Poly(I:C) (100 μg/ml) for 24 h, and the secretion of chemokines/cytokines was determined by ELISA. Error bars represent S.E.M. of three independent experiments. * P < 0.05. e HBEC3-KT cells were transfected as in d , lysed, and immunoblotted as indicated. f The indicated cells were lysed and immunoblotted for TLR3 and actin. Representative images of three independent experiments. g HBEC3-KT cells transfected with a control siRNA (siNS) or targeting c-FLIP (siFLIP), and were treated for 1 h with BV6 (5 μM). The cells were then exposed for 6 h to increasing doses of Poly(I:C), and the percentage of Annexin V+ cells was determined. Error bars represent S.E.M. of four independent experiments. * P < 0.05 and ** P < 0.01. h HBEC3-KT cells were treated as in f , lysed, and immunoblotted as indicated. i HBEC3-KT transfected with siNS or siFLIP were then exposed to Poly(I:C) (100 μg/ml) for the indicated times in presence or absence of BV6 (5 μM). The cells were then lysed and immunoblotted as indicated. Caspase cleavage products are indicated by arrowheads. Representative images of two independent experiments

Journal: Cell Death & Disease

Article Title: Release of c-FLIP brake selectively sensitizes human cancer cells to TLR3-mediated apoptosis

doi: 10.1038/s41419-018-0850-0

Figure Lengend Snippet: a Primary human bronchial epithelial cells (HBEC) were exposed to Poly(I:C) (100 μg/ml) for 24 h, and the secretion of chemokines/cytokines were determined. Error bars represent S.E.M. of two independent experiments. b Primary HBEC were exposed to Poly(I:C) (100 μg/ml) for 6 and 24 h (left panel), or to cisplatin for 24 h (right panel), and the percentage of Annexin V+ cells was determined by flow cytometry. Error bars represent S.E.M. of three (left panel) and two (right panel) independent experiments. c Human immortalized bronchial epithelial HBEC3-KT cells were treated with Poly(I:C) (100 μg/ml) for 6 h and 24 h (left panel), or to cisplatin (100 μM) for 24 h (right panel), and the percentage of Annexin V+ cells was determined. Error bars represent S.E.M. of four (left panel) and three (right panel) independent experiments. ** P < 0.01. d HBEC3-KT cells were transfected with a control non-silencing siRNA (siNS) or targeting TLR3 (siTLR3), exposed to Poly(I:C) (100 μg/ml) for 24 h, and the secretion of chemokines/cytokines was determined by ELISA. Error bars represent S.E.M. of three independent experiments. * P < 0.05. e HBEC3-KT cells were transfected as in d , lysed, and immunoblotted as indicated. f The indicated cells were lysed and immunoblotted for TLR3 and actin. Representative images of three independent experiments. g HBEC3-KT cells transfected with a control siRNA (siNS) or targeting c-FLIP (siFLIP), and were treated for 1 h with BV6 (5 μM). The cells were then exposed for 6 h to increasing doses of Poly(I:C), and the percentage of Annexin V+ cells was determined. Error bars represent S.E.M. of four independent experiments. * P < 0.05 and ** P < 0.01. h HBEC3-KT cells were treated as in f , lysed, and immunoblotted as indicated. i HBEC3-KT transfected with siNS or siFLIP were then exposed to Poly(I:C) (100 μg/ml) for the indicated times in presence or absence of BV6 (5 μM). The cells were then lysed and immunoblotted as indicated. Caspase cleavage products are indicated by arrowheads. Representative images of two independent experiments

Article Snippet: Primary HBEC cells were purchased from Lonza (Basel, Switzerland).

Techniques: Flow Cytometry, Transfection, Control, Enzyme-linked Immunosorbent Assay

a HBEC3-KT cells were treated with 250 nM paclitaxel (PTX) for 2 h, washed, and incubated with medium for 24 h. The cells were then exposed to increasing doses of Poly(I:C) for 6 h. Error bars represent S.E.M. of three independent experiments. b Analysis by western blot of c-FLIP and cIAP1/2 levels in HEBC3-KT cells treated with 250 nM PTX as in a . c Primary HBEC cells were treated with 250 nM PTX as in a , and then exposed 24 h later with increasing doses of Poly(I:C) for 6 h. One representative experiment out of two independent experiments is shown. i Analysis by western blot of c-FLIP and cIAP1/2 levels in primary HBEC cells treated with 250 nM PTX as in a

Journal: Cell Death & Disease

Article Title: Release of c-FLIP brake selectively sensitizes human cancer cells to TLR3-mediated apoptosis

doi: 10.1038/s41419-018-0850-0

Figure Lengend Snippet: a HBEC3-KT cells were treated with 250 nM paclitaxel (PTX) for 2 h, washed, and incubated with medium for 24 h. The cells were then exposed to increasing doses of Poly(I:C) for 6 h. Error bars represent S.E.M. of three independent experiments. b Analysis by western blot of c-FLIP and cIAP1/2 levels in HEBC3-KT cells treated with 250 nM PTX as in a . c Primary HBEC cells were treated with 250 nM PTX as in a , and then exposed 24 h later with increasing doses of Poly(I:C) for 6 h. One representative experiment out of two independent experiments is shown. i Analysis by western blot of c-FLIP and cIAP1/2 levels in primary HBEC cells treated with 250 nM PTX as in a

Article Snippet: Primary HBEC cells were purchased from Lonza (Basel, Switzerland).

Techniques: Incubation, Western Blot